DNA
lox66

Part:BBa_I718016:Design

Designed by: Eimad Shotar   Group: iGEM07_Paris   (2007-10-26)


lox66


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No modidification was made on the lox66 sequence


Source

This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.

Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)

You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:

http://2007.igem.org/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed

References